KissPeptin-10 Kit

Description

🧪 Product Profile: KissPeptin-10

Product Name: KissPeptin-10 (KP-10)
CAS Number: 692268-55-0
Category: Hypothalamic neuropeptide; GPR54 agonist
Form: Lyophilized white powder in amber glass vials
Molecular Formula: C₅₉H₈₉N₁₇O₁₄
Molecular Weight: 1302.46 g/mol
Purity: ≥98% (HPLC verified, TFA-free formulation)

🔬 Key Properties

• Amino Acid Sequence: YNWNSFGLRF-NH₂ (C-terminal decapeptide fragment of full-length kisspeptin-54)
• Solubility:
  • Water: ≥10 mg/mL (clear solution at pH 6.5-7.5)
  • PBS buffer: 13.8 mg/mL (10.6 mM at 25°C)
  • DMSO: Limited solubility (<2 mg/mL)
• Stability Profile:
  • Lyophilized: 24 months at -20°C; 12 months at 4°C (desiccated)
  • Reconstituted: 72 hours at 4°C; avoid repeated freeze-thaw cycles
• Biophysical Characteristics:
  • Isoelectric point (pI): 9.87
  • Secondary structure: Random coil in aqueous solution; α-helical propensity in membrane-mimetic environments

🧠 Mechanism of Action

KP-10 functions as the endogenous ligand for GPR54 (KISS1R), a Gq-coupled receptor, triggering:

• Intracellular Signaling: Phospholipase C activation → IP3/DAG pathway → Ca²⁺ mobilization (EC₅₀=0.3 nM)
• Reproductive Axis Activation: Stimulates GnRH neuron firing in the hypothalamic arcuate nucleus, leading to LH/FSH secretion
• Dose-Dependent Responses:
  • Low dose (1 nM): Pulsatile GnRH release (physiological pattern)
  • High dose (100 nM): Sustained gonadotropin secretion (pharmacological effect)
• Tissue Expression: Expressed in hypothalamus, pituitary, placenta, pancreas, and reproductive tissues

🧪 Research Applications

Widely used in reproductive neuroendocrinology and related fields:

• Puberty Studies: Induces precocious puberty in prepubertal rodents (5 nmol, ICV injection)
• Fertility Research: Restores gonadotropin secretion in hypogonadotropic hypogonadism models
• Cancer Biology: Investigates GPR54-mediated signaling in metastatic melanoma and breast cancer
• Metabolic Regulation: Modulates glucose homeostasis via pancreatic islet cell activation
• Neuroscience: Role in mediating stress-induced reproductive dysfunction (50 μg/kg, i.p. in chronic stress models)

⚙️ Handling & Administration

• Reconstitution Protocol:
  1. Add sterile water to achieve 1 mg/mL stock solution
  2. Dilute with assay buffer to working concentrations (typically 10-1000 nM)
  3. Adjust pH to 7.2 with 0.1M NaOH if precipitate forms
• Administration Routes:
  • Intracerebroventricular (ICV): 1-10 nmol in 2 μL artificial cerebrospinal fluid
  • Intraperitoneal (IP): 10-100 μg/kg in saline
  • In vitro: 10 nM-1 μM for cell culture studies
• Typical Dosing Regimens:
  • Acute studies: Single bolus injection
  • Chronic studies: Daily injections for 7-14 days (rodent models)

📦 Packaging & Quality Control

• Standard Packaging: 1 mg, 5 mg, and 10 mg vials with butyl rubber stoppers
• Quality Assurance:
  • Endotoxin level: ≤0.1 EU/mg
  • Amino acid analysis: Within ±5% of theoretical composition
  • Mass spectrometry: Confirmation of [M+H]⁺ = 1303.7 m/z
• Storage Configuration: Individually packaged with silica gel desiccant

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🧪 Biological Assays & Validation Data

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⚠️ Safety & Regulatory Considerations

• Hazard Classification: Not classified as hazardous (GHS 0) for research use
• Handling Precautions:
  • Use nitrile gloves and protective eyewear during reconstitution
  • Avoid inhalation of lyophilized powder (use biological safety cabinet)
• Regulatory Status: Research Use Only (RUO); not for human or veterinary therapeutic use
• Special Notes:
  • May interfere with reproductive studies in female rodents during estrous cycle
  • Requires serum-free media for in vitro studies (serum peptidases degrade KP-10)